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008			201210s2021 xxu| o |||| 0|eng d
020			_a9781071609972
024	7		_a10.1007/978-1-0716-0997-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK95.6 _b2021 EB
245	0	0	_aMolecular Plant Taxonomy : _bMethods and Protocols _cedited by Pascale Besse
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 400 páginas) _b78 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2222
505	0		_aPlant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives -- Guidelines for the Choice of Sequences for Molecular Plant Taxonomy -- Isolation and Purification of DNA from Complicated Biological Samples -- Herbarium Specimens: A Treasure for DNA Extraction, an Update -- Sequencing of Complete Chloroplast Genomes -- Utility of the Mitochondrial Genome in Plant Taxonomic Studies -- Nuclear Ribosomal RNA Genes: ITS Region -- Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla -- High-Throughput Genotyping Technologies in Plant Taxonomy -- Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny -- Development of Microsatellite Markers Using Next-Generation Sequencing -- Amplified Fragment Length Polymorphism: Applications and Recent Developments -- Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques -- Inter Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers -- Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity -- Introduction to Population Genomics Methods -- The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants -- Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization -- GISH: Resolving Interspecific and Intergeneric Hybrids.
520			_aThis fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an "integrative taxonomy" framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.
988			_aSpringer_Protocols_2021
650		7	_2embne _9168919 _aPlantas _xClasificación _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071609965
776	0	8	_iPrinted edition: _z9781071609989
776	0	8	_iPrinted edition: _z9781071609996
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0997-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI