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008			200926s2021 xxu| o |||| 0|eng d
020			_a9781071609897
024	7		_a10.1007/978-1-0716-0989-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC931 .O73 _b2021 EB
245	0	0	_aOsteoporosis and Osteoarthritis _cedited by Andre J. van Wijnen, Marina S. Ganshina
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 302 páginas) _b63 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2221
505	0		_aIsolation of Murine and Human Osteocyte -- Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells -- A Novel Enzymatic Digestion Approach for Isolation and Culture of Rodent Bone Marrow Mesenchymal Progenitors -- Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc -- Engineering Cartilage Tissue by Co-Culturing of Chondrocytes and Mesenchymal Stromal Cells -- Generation of Induced Pluripotent Stem Cells -- Specimen Preparation for Single Cell Sequencing Analysis of Skeletal Cells -- Mapping 5-Hydroxymethylcytosine (5hmC) Modifications in Skeletal Tissues Using High-Throughput Sequencing -- Using FRAP to Quantify Changes in Transcription Factor Dynamics after Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization -- Quantitative Molecular Models for Biological Processes: Modeling of Signal Transduction Networks with ANIMO -- Generation and Characterization of Mouse Models for Skeletal Disease -- Drill Hole Models to Investigate Bone Repair -- Generation and Experimental Outcomes of Closed Femoral Fracture in Mice -- Mouse Models of Osteoarthritis: Surgical Model of Post-Traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus -- Immunostaining of Skeletal Tissues -- Mapping Regional Cortical Bone Responses to Local Changes in Loading and Systemic Stimuli -- Pain and Activity Measurements.
520			_aThis detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration. The first half of the book examines cell and molecular biological aspects of osteoarthritis and osteoporosis, while the remainder of the collection focuses on in vivo models of skeletal tissue injury, degeneration, and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Osteoporosis and Osteoarthritis, Second Edition aims to assist investigators concerned with research topics broadly related to osteoporosis, osteoarthritis, intervertebral disc degeneration, as well as other musculoskeletal disorders.
988			_aSpringer_Protocols_2021
650		7	_2embne _9141478 _aOsteoporosis _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071609880
776	0	8	_iPrinted edition: _z9781071609903
776	0	8	_iPrinted edition: _z9781071609910
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0989-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI