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008			200925s2021 xxu| o |||| 0|eng d
020			_a9781071608845
024	7		_a10.1007/978-1-0716-0884-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB127 _b2021 EB
245	0	0	_aOpioid Receptors : _bMethods and Protocols _cedited by Santi M. Spampinato
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XV, 270 páginas) _b42 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2201
505	0		_aOverview of Genetic Analysis of Human Opioid Receptors -- Renilla luciferase Reporter Assay to Study 3'UTR-driven post-Transcriptional Regulation of OPRM1 -- Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1 -- Monitoring Opioid Receptors Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET) -- Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-visual Arrestins -- Functionalization and Bioconjugation of Nanoruby for Long-term, Ultrasensitive Imaging of Μ-Opioid Receptors.-- Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues -- Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells -- Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-tolerant Mice -- GTPgS-autoradiography for Studies of Opioid Receptor Functionality -- Evaluating Opioid Mediated Adenylyl Cyclase Inhibition in Live Cells using a BRET-based Assay -- Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury -- Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV -- An In Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion -- Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat -- The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls and Future Perspectives of Biased Agonists at Opioid Receptors -- Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment -- Evaluation of Murine Macrophage Cytokine Production after In Vivo Morphine Treatment -- Measurement of Macrophage Toll like Receptor 4 Expression After Morphine Treatment -- Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test -- Analysis of Opioid-Seeking Behavior through the Intravenous Self-administration Reinstatement Model in Rats -- Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice -- Early Life Stress as a Probe to Study the Opioid System in Developing Rodents -- Evaluation of m--opioid System Functionality in Mouse Pups: Ultrasonic Vocalizations as an Index Of Infant Attachment.
520			_aThis second edition presents an up-to-date chapters describing the most relevant and novel techniques employed to study the opioid receptors. Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid receptors, model systems to studying opiod receptor-mediated functions, and behavioral effects mediated by opioid receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Opioid Receptors: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2021
650		7	_2embne _9147178 _aOpiáceos _vManuales de laboratorio
650		7	_2embne _9170361 _aDolor _xTratamiento
776	0	8	_iPrinted edition: _z9781071608838
776	0	8	_iPrinted edition: _z9781071608852
776	0	8	_iPrinted edition: _z9781071608869
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0884-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI