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008			200921s2021 xxu| o |||| 0|eng d
020			_a9781071607954
024	7		_a10.1007/978-1-0716-0795-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRM281 _b2021 EB
245	0	0	_aVaccine Delivery Technology : _bMethods and Protocols _cedited by Blaine A. Pfeifer, Andrew Hill
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XV, 590 páginas) _b149 ilustraciones, 127 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2183
505	0		_aVaccine Delivery and Immune Response Basics -- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes -- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies -- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed -- Antigen Discovery in Bacterial Pan-Proteomes -- Alphavirus-Based Antigen Preparation -- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells -- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System -- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens -- Production of Zika Virus Virus-Like Particles -- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs) -- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines -- Whole-Cell Vaccine Preparation: Options and Perspectives -- O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein -- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines -- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines -- Attenuation Methods for Live Vaccines -- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism -- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells -- Lipofection-Based Delivery of DNA Vaccines -- Flavivirus DNA Vaccine Design and Adjuvant Selection -- Vaccine Delivery with a Detoxified Bacterial Toxin -- Needle-Less or Non-Invasive Delivery Technology -- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications -- A Hybrid Biological-Biomaterial Vector for Antigen Delivery -- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens -- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder -- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers -- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice -- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot -- The Measurement of Antigen-Specific IgG Titers by Direct ELISA -- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model -- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils -- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.
520			_aThis volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful field of vaccines. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2021
650		7	_2embne _9140409 _aVacunas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071607947
776	0	8	_iPrinted edition: _z9781071607961
776	0	8	_iPrinted edition: _z9781071607978
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0795-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI