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008			200918s2021 xxu| o |||| 0|eng d
020			_a9781071609583
024	7		_a10.1007/978-1-0716-0958-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQL957 _b2021 EB
245	0	0	_aEpigenetic Reprogramming During Mouse Embryogenesis : _bMethods and Protocols _cedited by Katia Ancelin, Maud Borensztein
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 318 páginas) _b74 ilustraciones, 58 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2214
505	0		_aEx Vivo Culture for Preimplantation Mouse Embryo to Analyse Pluripotency -- Microfabricated Device for High Resolution Imaging of Preimplantation Embryos -- In Vitro Culture of Mouse Blastocyst to the Egg Cylinder Stage via Mural Trophectoderm Excision -- Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces -- Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines -- Generation of Primordial Germ Cell-Like Cells in Small and Large Scales -- Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions -- Somatic Reprogramming by Nuclear Transfer -- Targeted Transgenic Mice using CRISPR/Cas9 Technology -- Whole-Mount Immunofluorescence Staining of Early Mouse Embryos -- Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization -- Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development -- Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-seq -- Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals -- Profiling DNA Methylation Genome-Wide in Single Cells -- Tracking Histone Modifications in Embryos and Low Input Samples using Ultrasensitive STAR ChIP-seq -- Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN -- DamID to Map Genome-Protein Interactions in Pre-Implantation Mouse Embryos -- Understanding Chromosome Structure during Early Mouse Development by Single-Cell Hi-C Analysis -- Bioinformatic Analysis of Single Cell Hi-C Data from Early Mouse Embryo.
520			_aThis volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9140065 _aEmbriología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071609576
776	0	8	_iPrinted edition: _z9781071609590
776	0	8	_iPrinted edition: _z9781071609606
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0958-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI