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008			200912s2021 xxu| o |||| 0|eng d
020			_a9781071608067
024	7		_a10.1007/978-1-0716-0806-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTA418.9 .N35 _b2021 EB
245	0	0	_aNanopore Technology : _bMethods and Protocols _cedited by Monifa A.V. Fahie
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 231 páginas) _b57 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2186
505	0		_aPreparation of Fragaceatoxin C (FraC) Nanopores -- Preparation of Cytolysin A (ClyA) Nanopores -- Building Synthetic Transmembrane Peptide Pores -- Design and Assembly of Membrane-Spanning DNA Nanopores -- Determining the Orientation of Porins in Planar Lipid Bilayers -- Revelation of Function and Inhibition of Wza through Single Channel Studies -- Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore -- Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules -- A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore -- Oligonucleotide-Directed Protein Threading through a Rigid Nanopore -- Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP -- Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel -- Free Energy Minimization for Vesicle Translocation through a Narrow Pore -- Single Ion-Channel Analysis in Droplet Interface Bilayer -- Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer -- Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.
520			_aThis detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.
988			_aSpringer_Protocols_2021
650		7	_2embne _9158454 _aMateriales nanoestructurados _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071608050
776	0	8	_iPrinted edition: _z9781071608074
776	0	8	_iPrinted edition: _z9781071608081
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0806-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI