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008			200611s2020 xxu| o |||| 0|eng d
020			_a9781071606766
024	7		_a10.1007/978-1-0716-0676-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP608 _b2020 EB
245	0	0	_aDynamin Superfamily GTPases : _bMethods and Protocols _cedited by Rajesh Ramachandran
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 221 páginas) _b53 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2159
505	0		_a Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae -- Purification of the Dynamin-related Protein Vps1 using Mammalian and Bacterial Expression Systems -- A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli -- Affinity Purification and Functional Characterization of Dynamin-related Protein 1 -- Purification and Characterization of MxB -- Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding -- Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-lipid Interactions -- Nucleotide-dependent Dimerization and Conformational Switching of Atlastin -- Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System -- Cell-free Analysis of Mitochondrial Fusion by Fluorescence Microscopy -- Electrophysiological Methods For Detection Of Membrane Leakage and Hemifission by Dynamin 1 -- Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore -- Cellular Assays for Measuring Dynamin Activity in Muscle Cells -- Measuring Drp1 Activity in Mitochondrial Fission in vivo -- Quantifying Drp1-mediated Mitochondrial Fission by Immunostaining in Fixed Cells -- Imaging Dynamin-related Protein 1 (Drp1)-mediated Mitochondrial Fission in Living Cells. .
520			_aThis volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2020
650		7	_2embne-DP _9146752 _aHidrolasas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071606759
776	0	8	_iPrinted edition: _z9781071606773
776	0	8	_iPrinted edition: _z9781071606780
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0676-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI