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020 _a9781071606032
024 7 _a10.1007/978-1-0716-0603-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK604.2 .M92
_b2020 EB
245 0 0 _aArbuscular Mycorrhizal Fungi :
_bMethods and Protocols
_cedited by Nuria Ferrol, Luisa Lanfranco
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XI, 257 páginas)
_b48 ilustraciones, 36 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2146
505 0 _aIsolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples -- The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants -- A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium -- Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization -- Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide -- Visualization of Arbuscular Mycorrhizal Fungal Extra-Radical Hyphae and Spores Vitality and Activity -- Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization -- Single-Spore-Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi -- High Throughput DNA Sequence-Based Analysis of AMF Communities -- Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP) -- Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal Fungi -- Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR -- Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis -- Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization -- Functional Analyses of Arbuscular Mycorrhizal Fungal Genes in Yeast -- Isotope Labelling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis -- Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae -- Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium Rhizogenes-Mediated Root Transformation in Medicago truncatula -- Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi.
520 _aThis book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbionts in agriculture more effectively.
988 _aSpringer_Protocols_2020
650 7 _2embne
_9146450
_aMicorrizas
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781071606025
776 0 8 _iPrinted edition:
_z9781071606049
776 0 8 _iPrinted edition:
_z9781071606056
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0603-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eu
_zSI