000			04574nam a22003975i 4500
999			_c391326 _d391326 _x1
001			391326
003			ES-MaUEC
005			20231212160847.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			180226s2018 xxu| o |||| 0|eng d
020			_a9781493976348
024	7		_a10.1007/978-1-4939-7634-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH434 _b2018 EB
245	0	0	_aBacterial Regulatory RNA : _bMethods and Protocols _cedited by Véronique Arluison, Claudio Valverde
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 395 páginas) _b84 ilustraciones, 62 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1737
505	0		_aWorkflow for a Computational Analysis of an sRNA Candidate in Bacteria -- Molecular Phylogenomics for the Identification and Characterization of trans-encoded Small Non-coding RNA Families -- Bioinformatic Approach for Prediction of CsrA/RsmA-regulating small RNAs in Bacteria -- Host-pathogen Transcriptomics by Dual RNA-seq -- Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing -- Assessment of External Guide Sequences' (EGS) Efficiency as Inducers of RNase P-mediated Cleavage of mRNA Target Molecules -- Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-dependent Termination of Transcription in vitro -- A Method to Map Changes in Bacterial Surface Composition Induced by Regulatory RNAs -- Fluorescence-based Methods for Characterizing RNA interactions in vivo -- Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay -- An Integrated Cell-free Assay to Study Translation Regulation by Small Bacterial Non-coding RNAs -- Quantitative Super-resolution Imaging of Small RNAs in Bacterial Cells -- Extraction and Analysis of RNA Isolated from Pure Bacteria-Derived outer Membrane Vesicles -- Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli -- High-resolution, High-throughput Analysis of Hfq Binding Sites using UV-crosslinking and Analysis of cDNA (CRAC) -- Producing Hfq/Sm Proteins and sRNAs for Structural and Biophysical Studies of Ribonucleoprotein Assembly -- Single Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing -- Techniques to Analyse sRNA-protein Cofactor Self-assembly in vitro -- Sequence-specific Affinity Chromatography of RNA-binding Proteins from Bacterial Cells -- Identification of Small RNA-protein Partners in Plant Symbiotic Bacteria -- A Modular Genetic System for High-throughput Profiling and Engineering of Multi-target Small RNAs. .
520			_aThis volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements in the rapidly emerging field of synthetic biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols: Methods and Protocol aims to be a guidebook to scientists, hoping to lead to the development of new tools and procedures for further developments in the field of sRNA biology. .
988			_aSpringer_Protocols_2018
650		7	_2embne _9140738 _aGenética microbiana _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493976331
776	0	8	_iPrinted edition: _z9781493976355
776	0	8	_iPrinted edition: _z9781493992515
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7634-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eu _zSI