000			04271nam a22003975i 4500
999			_c391319 _d391319 _x1
001			391319
003			ES-MaUEC
005			20240115092928.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			171123s2018 xxu| o |||| 0|eng d
020			_a9781493972951
024	7		_a10.1007/978-1-4939-7295-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTA164 _b2018 EB
245	0	0	_aSynthetic Metabolic Pathways : _bMethods and Protocols _cedited by Michael Krogh Jensen, Jay D. Keasling
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 354 páginas) _b68 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1671
505	0		_aParts characterization for tunable protein expression -- Enzyme nicotinamide cofactor specificity reversal guided by automated structural analysis and library design -- Bacterial genome editing strategy for control of transcription and protein stability -- An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization -- Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design -- Extended Metabolic Space Modeling -- Computational Methods to Assess the Production -- Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-gene Systems -- An adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism -- CRISPR-Cas9 toolkit for Actinomycete Genome Editing -- Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast using CasEMBLR -- A modified Gibson assembly method for cloning large DNA fragments with high GC contents -- Coupling Yeast Golden Gate and VEGAS for efficient assembly of the violacein pathway in Saccharomyces cerevisiae -- Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites For Phenotyping Of Microorganisms -- Selection of Highly Expressed Gene Variants in Escherichia coli using Translationally-coupled Antibiotic Selection Markers -- Design, Engineering, and Characterization Of Prokaryotic Ligand-binding Transcriptional Activators as Biosensors in Yeast -- A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers against Small Molecules -- High-throughput Microfluidics for the Screening Of Yeast Libraries -- Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution -- Two scale 13C Metabolic Flux Analysis for Metabolic Engineering.
520			_aThis volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Metabolic Pathways: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9140931 _aBiotecnología _xManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493972944
776	0	8	_iPrinted edition: _z9781493972968
776	0	8	_iPrinted edition: _z9781493984459
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7295-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eu _zSI