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008			100301s2009 xxu| o |||| 0|eng d
020			_a9781603272957
024	7		_a10.1007/978-1-60327-295-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623.5 .S63 _b2009 EB
245	0	0	_aTherapeutic Applications of RNAi : _bMethods and Protocols _cedited by John F. Reidhaar-Olson, Cristina M. Rondinone
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 148 páginas) _b18 ilustraciones, 6 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v555
505	0		_aDevelopment and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors -- Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery -- Liposomal siRNA for Ovarian Cancer -- Therapeutic Applications of RNAi for Silencing Virus Replication -- RNAi in the Malaria Vector, Anopheles gambiae -- RNAi Using a Chitosan/siRNA Nanoparticle System: In Vitro and In Vivo Applications -- Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease Through RNAi -- Studying Autoimmunity by In Vivo RNA Interference -- Transcriptional Gene Silencing Using Small RNAs -- Alternative Splicing as a Therapeutic Target for Human Diseases.
520			_aThe phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.
988			_aSpringer_Protocols_2009
650		7	_2embne _9144940 _aARN _xManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607611127
776	0	8	_iPrinted edition: _z9781617794940
776	0	8	_iPrinted edition: _z9781603272940
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-295-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eu _zSI