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020			_a9781071616055
024	7		_a10.1007/978-1-0716-1605-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2021 EB
245	0	0	_aFe-S Proteins : _bMethods and Protocols _cedited by Patricia C. Dos Santos
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 351 páginas) _b87 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2353
505	0		_aMolecular biology and genetic tools to investigate functional redundancy among Fe-S cluster carriers in E. coli -- Study Fe-S cluster proteins in Methanococcus maripaludis, a model archaeal organism -- Genetic approaches to uncover gene products involved in iron-sulfur protein maturation: High throughput genomic screening using transposon-sequencing -- Methods for heterologous overproduction of Fe-S proteins -- Enzymatic and Chemical in vitro Reconstitution of Iron-Sulfur Cluster Proteins -- Expression, isolation, and characterization of vanadium nitrogenase from Azotobacter vinelandii -- Ni-NTA affinity chromatography to characterize protein-protein interactions during Fe-S cluster biogenesis -- tRNA modifications as a readout of S and Fe-S metabolism -- Methods to unravel the roles of ATPases in Fe-S cluster biosynthesis -- Methods to investigate the kinetic profile of cysteine desulfurases -- A fast and ratiometric method for quantification of cysteine-bound persulfides based on alkylation and gel-shift assays -- Circular dichroism to probe the synthesis, transfer, and stability of Fe-S clusters -- Native mass spectrometry of iron-sulfur proteins -- Characterization of Paramagnetic Iron-Sulfur Clusters using Electron Paramagnetic Resonance Spectroscopy -- Characterization of Fe-S clusters in proteins by Mӧssbauer Spectroscopy -- Biochemical approaches to probe the role of the auxiliary iron-sulfur cluster of lipoyl synthase from Mycobacterium tuberculosis -- Methods to Screen for Radical SAM Enzyme Crystallization Conditions.
520			_aThis volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fe-S Proteins: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
988			_aSpringer_Protocols_2021
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781071616048
776	0	8	_iPrinted edition: _z9781071616062
776	0	8	_iPrinted edition: _z9781071616079
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1605-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI