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020 _a9781071615508
024 7 _a10.1007/978-1-0716-1550-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR82 .M5
_b2021 EB
245 0 0 _aStaphylococcus aureus :
_bMethods and Protocols
_cedited by Kelly C. Rice
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (X, 162 páginas)
_b34 ilustraciones, 25 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2341
505 0 _aMeasurement of Staphylococcus aureus Pigmentation by Methanol Extraction -- Analysis of Murein Hydrolases and Proteases through Zymography -- Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants -- Quantitative Hemolysis Assays -- In Vitro Assay for Quantifying Clumping of Staphylococcus aureus -- Measuring Staphylococcal Promoter Activities using a Codon-Optimized β-galactosidase Reporter -- Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve Assays -- Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity -- Quantification of Staphylococcus aureus Biofilm Formation by Crystal Violet and Confocal Microscopy -- Growth of Staphylococcus aureus using a Rotary Cell Culture System -- Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus -- Quantifying Staphylococcus aureus Membrane Potential using Flow Cytometry -- Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples -- A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins -- An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood -- Bone Marrow-Derived Macrophage Infection Assay -- Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins -- Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus Biofilm.
520 _aThis volume details the phenotypic characterization of Staphylococcus aureus, with a focus on in vitro and ex vivo methodologies. The chapters in this book cover topics such as in vitro assessment of classical S. aureus virulence attributes; quantifying promoter activity using a S. aureus codon-optimized lacZ plasmid; biologically-relevant growth environments; metabolic and stress resistance assays; and in vivo and ex vivo models of host-pathogen interaction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Staphylococcus aureus: Methods and Protocols is a valuable resource for anyone interested in this fascinating and developing field.
988 _aSpringer_Protocols_2021
650 7 _2embne
_9688498
_aStaphylococcus aureus
776 0 8 _iPrinted edition:
_z9781071615492
776 0 8 _iPrinted edition:
_z9781071615515
776 0 8 _iPrinted edition:
_z9781071615522
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1550-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
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