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008			230603s2021 xxu| o |||| 0|eng d
020			_a9781071608340
024	7		_a10.1007/978-1-0716-0834-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP624.5.M58 _b2023 EB
245	0	0	_aMitochondrial Gene Expression : _bMethods and Protocols _cedited by Michal Minczuk, Joanna Rorbach
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 333 páginas) _b59 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2192
505	0		_aIn vitro Analysis of mtDNA Replication -- In vivo Analysis of mtDNA Replication at the Single Molecule Level and with High Resolution -- In Vitro Reconstitution of Human Mitochondrial Transcription -- Investigating Mitochondrial Transcriptomes and RNA Processing using Circular RNA Sequencing -- Detection of 5-formylcytosine in Mitochondrial Transcriptome -- Visualization of Mitochondrial RNA Granules in Cultured Cells using 5-Bromouridine Labeling -- Quantitative Proteomics in Drosophila with Holidic Stable Isotope Labeling of Amino Acids in Fruit Flies (SILAF) -- Mass Spectrometric Analysis of Mitochondrial RNA Modifications -- mito-Ψ-Seq: A High-throughput Method for Systematic Mapping of Pseudouridine within Mitochondrial RNA -- High Throughput Detection of mtDNA Mutations Leading to tRNA Processing Errors -- High-throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells -- RNA Crosslinking to Analyze the Mitochondrial RNA-binding Proteome -- Visualizing Mitochondrial Ribosomal RNA and Mitochondrial Protein Synthesis in Human Cell Lines -- Mitoribosome Profiling From Human Cell Culture - A High Resolution View of Mitochondrial Translation -- Application of cryo-EM for Visualization of Mitoribosomes -- Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes -- The Analysis of Yeast Mitochondrial Translation -- In situ Studies of Mitochondrial Translation by Cryoelectron Tomography -- Mitochondrial Complexome Profiling -- Blue Native Electrophoresis to Study the Oxphos Complexes -- Identification of Putative Mitochondrial Protease Substrates. .
520			_aThis volume details the most recent advancements in the field of mitochondrial gene expression. Chapters guide readers through methods and protocols on mtDNA replication, transcription, and translation to membrane insertion of the mtDNA-encoded protein products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines. Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2021
650		7	_2embne _9670608 _aADN mitocondrial
650		7	_2embne _9162770 _aExpresión génica
776	0	8	_iPrinted edition: _z9781071608333
776	0	8	_iPrinted edition: _z9781071608357
776	0	8	_iPrinted edition: _z9781071608364
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0834-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI