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020			_a9781071610480
024	7		_a10.1007/978-1-0716-1048-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK625 .M7 _b2021 EB
245	0	0	_aTrichoderma reesei : _bMethods and Protocols _cedited by Astrid R. Mach-Aigner, Roland Martzy
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 364 páginas) _b63 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2234
505	0		_aEcological Genomics and Evolution of Trichoderma reesei -- Industrial Relevance of Trichoderma reesei as an Enzyme Producer -- The Potential of Synthetic Biology for Trichoderma reesei -- Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei -- Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei -- Open the Pores: Electroporation for the Transformation of Trichoderma reesei -- Sexual Crossing of Trichoderma reesei -- CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei -- The Copper-Controlled RNA Interference System in Trichoderma reesei -- Batch Cultivation of Trichoderma reesei -- Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations -- Measurement of Cellulase and Xylanase Activities in Trichoderma reesei -- Flow Cytometry for Filamentous Fungi -- Molecular Identification of Trichoderma reesei -- In Vivo Footprinting Analysis in Trichoderma reesei -- RNA Characterization in Trichoderma reesei -- Proteomic Profiling of the Secretome of Trichoderma reesei -- Transcriptomics in Trichoderma reesei -- The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances -- In Silico Gene Analysis and Oligonucleotide Design for Construction of Expression Vectors -- PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a -- TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations.
520			_aThis detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trichoderma reesei: Methods and Protocols serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.
988			_aSpringer_Protocols_2021
650		7	_2embne _9138683 _aHongos
650		7	_2embne _9672737 _aBiología sintética
776	0	8	_iPrinted edition: _z9781071610473
776	0	8	_iPrinted edition: _z9781071610497
776	0	8	_iPrinted edition: _z9781071610503
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1048-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI