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008			230530s2021 xxu| o |||| 0|eng d
020			_a9781071608685
024	7		_a10.1007/978-1-0716-0868-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.27.Y43 _b2021 EB
245	0	0	_aYeast Protocols _cedited by Wei Xiao
250			_a4th edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 260 páginas) _b55 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2196
505	0		_a Use of Yeast Plasmids - Transformation and Inheritance Assays -- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae -- CRISPR Nickase-Mediated Base Editing in Yeast -- Genomic Promoter Shuffling by Using Recyclable Cassettes -- Study Essential Gene Functions by Plasmid Shuffling -- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae -- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae -- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections -- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe -- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map -- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly -- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE -- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering -- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9 -- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA -- Assays for Autophagy III: Observing Dynamic Protein Trafficking -- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification -- High-Throughput Rapid Yeast Chronological Lifespan Assay -- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast -- Monitoring 5'-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis. .
520			_aThis fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9163872 _aLevaduras (Botánica) _xBiotecnologia
776	0	8	_iPrinted edition: _z9781071608678
776	0	8	_iPrinted edition: _z9781071608692
776	0	8	_iPrinted edition: _z9781071608708
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0868-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI