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020 _a9781071609286
024 7 _a10.1007/978-1-0716-0928-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP552 .P6
_b2021 EB
245 0 0 _aPolypeptide Materials :
_bMethods and Protocols
_cedited by Maxim G. Ryadnov
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XIV, 268 páginas)
_b80 ilustraciones, 66 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2208
505 0 _aA Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach -- SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification -- Peptide Nanoparticles for Gene Packaging and Intracellular Delivery -- Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung -- FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures -- Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes -- Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides -- Electrostatic Self-Assembly of Protein Cage Arrays -- Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity -- Imaging and 3D Reconstruction of De Novo Peptide Capsids -- Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra -- Forming Low Molecular Weight Hydrogels by Electrochemical Methods -- In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study -- Measurement of Peptide Coating Thickness and Chemical Composition Using XPS -- Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy -- Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials -- Ultramicrotomy Analysis of Peptide-Treated Cells.
520 _aThis book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.
988 _aSpringer_Protocols_2021
650 7 _2embne
_9146243
_aPéptidos
650 7 _2embne
_9139861
_aProteínas
776 0 8 _iPrinted edition:
_z9781071609279
776 0 8 _iPrinted edition:
_z9781071609293
776 0 8 _iPrinted edition:
_z9781071609309
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0928-6
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eIG
_zSI