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008			230530s2020 xxu| o |||| 0|eng d
020			_a9781071606070
024	7		_a10.1007/978-1-0716-0607-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.24 _b2020 EB
245	0	0	_aExperimental Protocols in Biotechnology _cedited by Neelima Gupta, Varsha Gupta
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XV, 246 páginas) _b79 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aChapter 1. Enzyme Linked Immuno sorbent Assay Detection of Bacterial Wilt Causing Ralstonia solanacearum -- Chapter 2. Diagnosis of Tropical Fascioliasis using Specific Antigen Detection in Faeces of Infected Human Being or Animal Species -- Chapter 3. CMV Promoter‐Driven Expression and Visualization of Tagged Proteins in Live and Fixed Zebrafish Embryonic Epidermis -- Chapter 4. Detection of Blood Parasites and Estimation of Haematological Indices in Fish -- Chapter 5. Techniques to Conduct Morphological and Molecular Investigations of Nematodes -- Chapter 6. Computational Approaches in Drug Designing and its Application -- Chapter 7. Bacteriophage Control for Pseudomonas Aeruginosa Biofilm Formation and Eradication -- Chapter 8 -- Detection of Phospho-proteins (phospho-serine and phospho-threonine) from Thylakoid Membranes using Western Blotting -- Chapter 9. Estimation of Plasma membrane and Tonoplast ATPase activity in Plant Tissues -- Chapter 10. Quantification of Conserved MicroRNA in Plants and Validation of New Targets -- Chapter 11. Isolation of Natural Killer Cells from Mouse Bone Marrow and Spleen -- Chapter 12. Gene Expression Analysis from Human Peripheral Blood Mononuclear Cells -- Chapter 13. Use of Droplet PCR in Biomedical Research -- Chapter 14. Determination of Michaelis-Menten Enzyme Kinetics parameters of Alkaline Phosphatase in clinical sample -- Chapter 15. Novel Technique for the Detection of LncRNAs on Tissue Sections.
520			_aThis manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. .
988			_aSpringer_Protocols_2020
650		7	_2embne _9140931 _aBiotecnología
650		7	_2embne _9140525 _aÁcidos nucleicos
776	0	8	_iPrinted edition: _z9781071606063
776	0	8	_iPrinted edition: _z9781071606087
776	0	8	_iPrinted edition: _z9781071606094
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0607-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI