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008			230529s2020 xxu| o |||| 0|eng d
020			_a9781071606230
024	7		_a10.1007/978-1-0716-0623-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH452.8 _b2020 EB
245	0	0	_aIn Situ Hybridization Protocols _cedited by Boye Schnack Nielsen, Julia Jones
250			_a5th edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIII, 392 páginas) _b91 ilustraciones, 90 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2148
505	0		_aGuidelines for the Optimization and Validation of In Situ Hybridization -- Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization -- Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories -- Fluorescent In Situ Hybridization using Oligonucleotide-Based Probes -- Visualizing Genome Reorganization using 3D DNA FISH -- MicroRNA In Situ Hybridization in Paraffin Embedded Cultured Cells -- Multiplexed Detection and Analysis of Low Abundant Abundance Long Non-Coding RNA using RNAscopeTM in Cultured Cells -- Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0) -- Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0) -- Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution within Whole-Mount Vertebrate Embryos: qHCR and dHCR imaging (v3.0) -- Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues -- RNAscopeTM Multiplex Detection in Zebrafish -- Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues using RNAscopeTM -- Automated ISH for Validated Histological Mapping of Lowly Expressed Genes -- Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping -- Automated Co-In Situ Hybridization and Immunofluorescence using Archival Tumor Tissue -- Automated 5-Color Multiplex Co-Detection of MicroRNA and Protein Expression in Fixed Tissue Specimens -- Mixed Multiplex Staining: Automated RNAscopeTM and OPALTM for Multiple Targets -- Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscopeTM In Situ Hybridization and Immunofluorescence Protocol -- In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections -- GeoMx™ RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue -- In Situ Point Mutation Detection in FFPE Colorectal Cancers using the BaseScope Assay -- Using In Situ Padlock Probe Technology to Detect mRNA Splice Variants in Tumor Cells -- Automated One-Double-Z Pair BaseScope™ for CircRNA In Situ Hybridization.
520			_aThis fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field. .
988			_aSpringer_Protocols_2020
650		7	_2embne _9688531 _aHibridación in situ
776	0	8	_iPrinted edition: _z9781071606223
776	0	8	_iPrinted edition: _z9781071606247
776	0	8	_iPrinted edition: _z9781071606254
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0623-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI