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020 _a9781071602072
024 7 _a10.1007/978-1-0716-0207-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR185.8.T2
_b2020 EB
245 0 0 _aMAIT Cells :
_bMethods and Protocols
_cedited by Helen Kaipe, Isabelle Magalhaes
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XII, 310 páginas)
_b54 ilustraciones, 41 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2098
505 0 _aMAIT Cells in Health and Disease -- Isolation and Characterization of MAIT Cells from Human Tissue Biopsies -- Isolation and Characterization of MAIT Cells from Tumor Tissues -- Isolation of Immune Cells from Placental Tissues and Phenotypic and Functional Analysis of MAIT Cells -- Methods for High-Dimensional Flow Cytometry Analysis of Human MAIT Cells in Tissues and Peripheral Blood -- In Situ Detection of MAIT Cells and MR1 Expressing Cells in Tissue Biopsies Utilizing Immunohistochemistry -- Human MAIT Cell Activation In Vitro -- Interactions between MAIT Cells and Dendritic Cells -- Influenza A Virus-Infected Lung Epithelial Cell Co-Culture with Human Peripheral Blood Mononuclear Cells -- Quantification of Human MAIT Cell-Mediated Cellular Cytotoxicity and Antimicrobial Activity -- MAIT Cell Activation by Fungal Pathogens -- A Flow Chamber Assay for Studying MAIT Cell Trafficking -- Production of MR1 Tetramers Loaded with Microbial Ligands -- Immune Monitoring of Human Mucosal Associated Invariant T Cells by Quantitative Proteomics -- Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells -- Reprogramming of MAIT Cells to Pluripotency and Redifferentiation -- Study of MAIT Cell Activation in Viral Infections In Vivo -- MAIT Cells in Type 1 Diabetes Mouse Models -- Magnetic-Bead Based Enrichment of Murine MAIT Cells.
520 _aThis volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers methods to isolate and characterize MAIT cells from human tissues including liver, colon tumors, placenta and decidua, and endometrium. Part Two discusses MR1 independent stimulation, and looks at the activation of MAIT cells by different stimulatory agents. Part Three describes various methods to develop tools to analyze, monitor, and generate MAIT cells in vitro, including the production of MR1-tetramers and how they can be loaded with bacterial antigens and used for the detection of MAIT cells. The last part covers the use of murine models to study MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, MAIT Cells: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field. Chapters 9 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988 _aSpringer_Protocols_2020
650 7 _2embne
_9144344
_aLinfocitos T
650 7 _2embne
_9138330
_aInmunología
776 0 8 _iPrinted edition:
_z9781071602065
776 0 8 _iPrinted edition:
_z9781071602089
776 0 8 _iPrinted edition:
_z9781071602096
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0207-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
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998 _b05/2023
_dz
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_zSI