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020 _a9781071602034
024 7 _a10.1007/978-1-0716-0203-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRM276
_b2020 EB
245 0 0 _aCell Reprogramming for Immunotherapy :
_bMethods and Protocols
_cedited by Samuel G. Katz, Peter M. Rabinovich
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XII, 343 páginas)
_b41 ilustraciones, 34 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2097
505 0 _aT Cell Reprogramming against Cancer -- Identification of Cell Surface Targets for CAR T Cell Immunotherapy -- Microfluidic Approach for Highly Efficient Viral Transduction -- Single-cell Cytokine Analysis to Characterize CAR-T Cell Activation -- Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells -- Engineering of Natural Killer Cells for Clinical Application -- Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells -- In vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection -- Immunomodulation of NK Cell Activity -- An Overview of Advances in Cell-based Cancer Immunotherapies Based on the Multiple Immune-cancer Cell Interactions -- Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy -- Reprogramming Exosomes for Immunotherapy -- Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment -- CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow Derived Macrophages -- Genome-Wide CRISPRi/a Screening in an In Vitro Co-culture Assay of Human Immune Cell with Tumor Cells -- The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV) -- Radiolabeling and Imaging of Adoptively Transferred Immune Cells by Positron Emission Tomography -- Functional Analysis of Human Hematopoietic Stem Cells in vivo in Humanized Mice -- Monitoring Allogeneic CAR-T Cells using Flow Cytometry -- Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma -- Place of Academic GMP Facilities in Modern Cell Therapy. .
520 _aThis volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988 _aSpringer_Protocols_2020
650 7 _2embne
_9138330
_aInmunología
650 7 _2embne
_9139477
_aCitología
650 7 _2embne
_9145440
_aInmunoterapia
776 0 8 _iPrinted edition:
_z9781071602027
776 0 8 _iPrinted edition:
_z9781071602041
776 0 8 _iPrinted edition:
_z9781071602058
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0203-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eIG
_zSI