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020 _a9781493997909
024 7 _a10.1007/978-1-4939-9790-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK753 .N54
_b2020 EB
245 0 0 _aNitrogen Metabolism in Plants :
_bMethods and Protocols
_cedited by Kapuganti Jagadis Gupta
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publishing
_c2020
300 _a1 recurso en línea (X, 178 páginas)
_b41 ilustraciones, 32 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2057
505 0 _aAn Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies.
520 _aThis volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.
988 _aSpringer_Protocols_2020
650 7 _2embne
_9182353
_aNitrógeno
_xMetabolismo
650 7 _2embne
_9138492
_aBotánica
776 0 8 _iPrinted edition:
_z9781493997893
776 0 8 _iPrinted edition:
_z9781493997916
776 0 8 _iPrinted edition:
_z9781493997923
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9790-9
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eIG
_zSI