000			04982nam a22004095i 4500
999			_c391224 _d391224 _x1
001			391224
003			ES-MaUEC
005			20231212160836.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			230527s2020 xxu| o |||| 0|eng d
020			_a9781493998180
024	7		_a10.1007/978-1-4939-9818-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981 _b2020 EB
245	0	0	_aPlant Meiosis : _bMethods and Protocols _cedited by Mónica Pradillo, Stefan Heckmann
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XIV, 405 páginas) _b76 ilustraciones, 66 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2061
505	0		_aA Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for "-omics" Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes - EdU as Compound Example.
520			_aThis volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. .
988			_aSpringer_Protocols_2020
650		7	_2embne _9139640 _aGenética vegetal
650		7	_2embne _9138492 _aBotánica
776	0	8	_iPrinted edition: _z9781493998173
776	0	8	_iPrinted edition: _z9781493998197
776	0	8	_iPrinted edition: _z9781493998203
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9818-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI