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008			230527s2020 xxu| o |||| 0|eng d
020			_a9781493998371
024	7		_a10.1007/978-1-4939-9837-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR182.2 .A54 _b2020 EB
245	0	0	_aTransgenic Mouse : _bMethods and Protocols _cedited by Melissa A. Larson
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 236 páginas) _b45 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2066
505	0		_aThe History of Transgenesis -- Pronuclear Microinjection of One-Cell Embryos -- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection -- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos -- Targeted Mutations in the Mouse via Embryonic Stem Cells -- Blastocyst Microinjection with Embryonic Stem Cells -- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos -- Embryo Transfer Surgery -- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device -- Nuclear Transfer and Cloning -- Sperm-Mediated Genetic Modifications -- Genotyping Genetically Modified (GM) Mice -- Nomenclature: Naming Your Gene-Modified Mouse -- Breeding Strategies for Genetically Modified Mice -- Strategies for Behaviorally Phenotyping the Transgenic Mouse -- Cryobanking and Recovery of Genetically Modified Mice -- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques -- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.
520			_aThis volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. .
988			_aSpringer_Protocols_2020
650		7	_2embne _9138513 _aGenética animal
650		7	_2embne _9138515 _aAnimales de laboratorio
776	0	8	_iPrinted edition: _z9781493998364
776	0	8	_iPrinted edition: _z9781493998388
776	0	8	_iPrinted edition: _z9781493998395
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9837-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI