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008			230527s2018 xxu| o |||| 0|eng d
020			_a9781493986958
024	7		_a10.1007/978-1-4939-8695-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR92 .P75 _b2018 EB
245	0	0	_aMicrobial Proteomics : _bMethods and Protocols _cedited by Dörte Becher
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XV, 344 páginas) _b50 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1841
505	0		_aFilter-Aided Sample Preparation for Proteome Analysis -- Protein Enrichment from Highly Dilute Samples with StrataClean -- Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins -- Enrichment of Cell Surface-Associated Proteins in Gram+ Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis -- Preparation of Bacterial Magnetosomes for Proteome Analysis -- Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics -- Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS -- Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms -- Proteomic Signatures in Staphylococcus aureus -- How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis -- Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE -- Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses -- Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N) -- Next-Generation Trapping of Protease Substrates by Label-Free Proteomics -- In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings -- Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography -- Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation -- Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria -- Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions -- Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies -- Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter -- Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses.
520			_aThis detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond.
988			_aSpringer_Protocols_2018
650		7	_2embne _9140738 _aGenética microbiana
776	0	8	_iPrinted edition: _z9781493986934
776	0	8	_iPrinted edition: _z9781493986941
776	0	8	_iPrinted edition: _z9781493993680
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8695-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI