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008			230525s2018 xxu| o |||| 0|eng d
020			_a9781493986750
024	7		_a10.1007/978-1-4939-8675-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR76.5 _b2018 EB
245	0	0	_aBacterial Chromatin : _bMethods and Protocols _cedited by Remus T. Dame
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 419 páginas) _b118 ilustraciones, 81 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1837
505	0		_aDetermination of the 3D Genome Organization of Bacteria using Hi-C -- Overview of Processing and Analyzing Hi-C Data on Bacteria -- GeF-seq：A Simple Procedure for Base Pair Resolution ChIP-seq -- Genomic SELEX Screening of Regulatory Targets of Escherichia coli Transcription Factors -- Modular assembly of Synthetic Secondary Chromosomes -- High-resolution Characterization of DNA/Protein Complexes in Living Bacteria -- Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-dimensional Superresolution Structured Illumination Microscopy -- Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-associated Proteins in Escherichia coliAtomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization -- Dynamic Light Scattering of DNA-ligand Complexes -- Microscale Thermophoresis Analysis of Chromatin Interactions -- Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins -- Approaches for Determining DNA Persistence Length using Atomic Force Microscopy -- Quantitation of DNA Binding Affinity using Tethered Particle Motion -- Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-stretching with Single-molecule Imaging -- Unravelling the Biophysical Properties of Chromatin Proteins and DNA using Acoustic Force Spectroscopy -- Unravelling DNA organization with single-molecule Force Spectroscopy using Magnetic Tweezers -- In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase -- Deciphering 3D Organization Of Chromosomes using Hi-C Data -- Molecular Dynamics Simulation of a Feather-boa Model of Bacterial Chromosome. .
520			_aThis volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9144121 _aCromatina
776	0	8	_iPrinted edition: _z9781493986743
776	0	8	_iPrinted edition: _z9781493986767
776	0	8	_iPrinted edition: _z9781493993628
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8675-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI