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008			230525s1994 xxu| o |||| 0|eng d
020			_a9781592595099
024	7		_a10.1385/0896032329 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.9 .S6 _b1994 EB
100	1		_aJones, Christopher _d1954- _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688517
245	1	0	_aMicroscopy, Optical Spectroscopy, and Macroscopic Techniques _cby Christopher Jones, Barbara Mulloy, Adrian H. Thomas
250			_a1st edition 1994
264		1	_aTotowa, NJ _bHumana Press _c1994
300			_a1 recurso en línea (XI, 251 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v22
505	0		_aMicroscopy -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Visualization of Unshadowed DNA by Electron Microscopy -- Biological Applications of Scanning Tunneling Microscopy -- Scattering And SedimentatioN -- High-Flux X-Ray and Neutron Solution Scattering -- Determination of Macromolecular Homogeneity, Shape, and Interactions Using Sedimentation Velocity Analytical Ultracentrifugation -- Determination of Absolute Molecular Weights Using Sedimentation Equilibrium Analytical Ultracentrifugation -- Classical Light Scattering for the Determination of Absolute Molecular Weights and Gross Conformation of Biological Macromolecules -- Determination of Diffusion Coefficients of Biological Macromolecules by Dynamic Light Scattering -- Calorimetric Methods -- to Microcalorimetry and Biomolecular Energetics -- Differential Scanning Calorimetry -- Isothermal Titration Microcalorimetry -- Optical Spectroscopy -- Optical Spectroscopy -- The Measurement of Electronic Absorption Spectra in the Ultraviolet and Visible -- Analysis of Polypeptide and Protein Structures Using Fourier Transform Infrared Spectroscopy -- Fluorescence Spectroscopy -- Circular Dichroism.
520			_aThis is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec­ troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand­ ing for the biochemist or biologist who needs to collaborate with spe­ cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol­ vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc­ ture using circular dichroism and Fourier-transform infrared spec­ troscopy.
988			_aSpringer_Protocols_1994
650		7	_2embne _9688518 _aEspectroscopios
700	1		_aMulloy, Barbara _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688519
700	1		_aThomas, Adrian H. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688520
776	0	8	_iPrinted edition: _z9781489940018
776	0	8	_iPrinted edition: _z9780896032323
776	0	8	_iPrinted edition: _z9781489940001
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032329 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b05/2023 _dz _eIG _zSI