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020			_a9781071612668
024	7		_a10.1007/978-1-0716-1266-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB147.5 _b2021 EB
245	0	0	_aMitochondrial Medicine _nVolume 2, _pAssessing Mitochondria _cedited by Volkmar Weissig, Marvin Edeas
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising : _bImprint: Springer, _c2021
300			_a1 recurso en línea (XVI, 459 páginas) _b112 ilustraciones, 75 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2276
505	0		_aMitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow -- Preparation of 'Functional' Mitochondria - A Challenging Business -- Isolation and Quality Control of Functional Mitochondria -- Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient -- Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues -- Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems -- Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes -- Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging -- Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays -- Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues -- Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria -- Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence -- Assessment of Mitochondrial Ca2+ Uptake -- Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices -- Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis -- Analysis of Mitochondrial Dysfunction during Cell Death -- Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes -- Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane -- Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway -- 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction -- Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology -- High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria -- Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism -- Fluorescence-based Assay for Measuring OMA1 Activity -- Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay -- Extraction of Functional Mitochondria Based on the Membrane Stiffness -- A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing -- A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry -- In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans -- Assessing Impact of Platinum Complexes on Mitochondrial Functions -- In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models -- Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a New Molecular Biosensor.
520			_aThis second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional' mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.
988			_aSpringer_Protocols_2021
650		7	_2embne _9670608 _aADN mitocondrial
650		7	_2embne _9147980 _aMitocondrias
700	1		_aWeissig, Volkmar _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aEdeas, Marvin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071612651
776	0	8	_iPrinted edition: _z9781071612675
776	0	8	_iPrinted edition: _z9781071612682
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1266-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI