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020			_a9781071607831
024	7		_a10.1007/978-1-0716-0783-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH324.9.C7 _b2021 EB
245	0	0	_aCryopreservation and Freeze-Drying Protocols _cedited by Willem F. Wolkers, Harriëtte Oldenhof
250			_a4th edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XVI, 742 páginas) _b156 ilustraciones, 104 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2180
505	0		_aPrinciples Underlying Cryopreservation and Freeze-Drying of Cells and Tissues -- Principles of Ice-Free Cryopreservation by Vitrification -- The Principles of Freeze-Drying and Application of Analytical Technologies -- Mathematical Modeling and Optimization of Cryopreservation in Single Cells -- Mathematical Modeling of Protectant Transport in Tissues -- Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation -- Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics -- High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics -- Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization -- DSC Analysis of Thermophysical Properties for Biomaterials and Formulations -- Osmometric Measurements of Cryoprotective Agent Permeation into Tissues -- Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes -- Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research -- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements -- Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm -- Cryopreservation of Avian Semen -- Cryopreservation of Mouse Sperm for Genome Banking -- Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages -- Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent -- Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage -- Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen -- Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research -- Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices -- Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts -- Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration -- Frozen Blood Reserves -- Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes -- Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells -- Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells -- Protocol for Cryopreservation of Endothelial Monolayers -- Vitrification of Heart Valve Tissues -- Cryopreservation of Algae -- Cryopreservation of Fern Spores and Pollen -- Cryopreservation of Plant Cell Lines Using Alginate Encapsulation -- Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3 -- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species -- Freeze-Drying of Proteins -- Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties -- Preservation of Mammalian Sperm by Freeze-Drying -- Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability.
520			_aThis fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution. The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2021
650		7	_2embne _9673643 _aCrioconservación de órganos, tejidos, etc.
650		7	_2embne _9688493 _aLiofilización
776	0	8	_iPrinted edition: _z9781071607824
776	0	8	_iPrinted edition: _z9781071607848
776	0	8	_iPrinted edition: _z9781071607855
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0783-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b05/2023 _dz _eIG _zSI