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008			230520s2021 xxu| o |||| 0|eng d
020			_a9781071607435
024	7		_a10.1007/978-1-0716-0743-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2021 EB
245	0	0	_aRNA Abundance Analysis : _bMethods and Protocols _cedited by Hailing Jin, Isgouhi Kaloshian
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 233 páginas) _b12 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2170
505	0		_aCRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana -- Strand-Specific RNA-Seq Applied to Malaria Samples -- Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning -- Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting -- Quantitative Analysis of Plant miRNA Primary Transcripts -- A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq -- Analysis of RBP Regulation and Co-Regulation of mRNA 3' UTR Regions in a Luciferase Reporter System -- Extraction of Small RNAs by Titanium Dioxide Nanofibers -- Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data -- Purification and Analysis of Chloroplast RNAs in Arabidopsis -- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes -- Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs -- Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses -- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue -- Studying RNA-Protein Interaction Using Riboproteomics -- Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation.
520			_aThis updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
988			_aSpringer_Protocols_2021
650		7	_2embne _9144940 _aARN
650		7	_2embne _9138639 _aBioquímica
776	0	8	_iPrinted edition: _z9781071607428
776	0	8	_iPrinted edition: _z9781071607442
776	0	8	_iPrinted edition: _z9781071607459
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0743-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI