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008			230520s2021 xxu| o |||| 0|eng d
020			_a9781071608142
024	7		_a10.1007/978-1-0716-0814-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP751 _b2021 EB
245	0	0	_aLipid Rafts : _bMethods and Protocols _cedited by Erhard Bieberich
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 350 páginas) _b79 ilustraciones, 62 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2187
505	0		_aIsolation and Analysis of Lipid Rafts from Neural Cells and Tissues -- A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study -- Biochemical Analysis of Lipid Rafts to Study Pathogenic Mechanisms of Neural Diseases -- Amyloid- β Interactions with Lipid Rafts in Biomimetic Systems: A Review of Laboratory Methods -- Extracellular Vesicles Containing Ceramide-Rich Platforms: "Mobile Raft" Isolation and Analysis -- Isolation of Lipid Rafts (Detergent-Resistant Microdomains) and Comparison to Extracellular Vesicles (Exosomes) -- Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics -- Association of Glycolipids and Growth Factor Receptors in Lipid Rafts -- Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts during Apoptosis Triggering -- Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach -- Analysis of Lipids in Ceramide-Enriched Membrane Domains -- Chemotherapeutic Agents-Induced Ceramide Rich Platforms (CRPs) in Endothelial Cells and their Modulation -- Biophysical Analysis of Lipid Domains by Fluorescence Microscopy -- Biophysical Analysis of Lipid Domains in Mammalian and Yeast Membranes by Fluorescence Spectroscopy -- Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy -- Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components -- Super-Resolution Microscopy of Sphingolipids -- Detection of Functionalized Sphingolipid Analogues in Detergent Resistant Membranes of the Immune Cells -- Immunofluorescence Labeling of Lipid-Binding Proteins CERTs to Monitor Lipid Rafts Dynamics -- Cross-Link/Proximity Ligation Assay for Visualization of Lipid and Protein Complexes in Lipid Rafts.
520			_aThis volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9142933 _aLípidos
776	0	8	_iPrinted edition: _z9781071608135
776	0	8	_iPrinted edition: _z9781071608159
776	0	8	_iPrinted edition: _z9781071608166
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0814-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI