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008			200717s2020 xxu| o |||| 0|eng d
020			_a9781071606803
024	7		_a10.1007/978-1-0716-0680-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623.8 .P75 _b2020 EB
245	0	0	_aRNA-Chromatin Interactions : _bMethods and Protocols _cedited by Ulf Andersson Vang Ørom
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 266 páginas) _b54 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2161
505	0		_aTuning the Expression of Long Noncoding RNA loci with CRISPR Interference -- Identification of Chromatin binding sites for Long noncoding RNAs by Chromatin Oligo Affinity Precipitation (ChOP) -- Knock-down of Nuclear lncRNAs by Locked Nucleic Acid (LNA) Gapmers in Nephron Progenitor Cells -- Design and Application of a Short (16-mer) Locked Nucleic Acid splice-Switching Oligonucleotide for Dystrophin Production in Duchenne Muscular Dystrophy Myotubes -- Targeting Polyadenylation for Retention of RNA at Chromatin -- Simultaneous Detection of RNAs and Proteins with Subcellular Resolution -- In Vivo Crosslinking of Histone and RNA-binding Proteins -- Proteomics-based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA -- 2D Saturation Transfer Difference NMR for Determination of Protein Binding Sites on RNA Guanine Quadruplexes -- Mapping Transcriptome-wide and Genome-wide RNA-DNA Contacts with Chromatin-Associated RNA Sequencing (ChAR-seq) -- Identification of cis-elements for RNA Subcellular Localization through REL-seq -- Transcriptome-wide Mapping of Protein-RNA Interactions -- Proximity RNA-seq, A Sequencing Method to Identify Co-localization of RNA -- Immunoprecipitation of DNA-RNA Hybrids using the S9.6 Antibody -- Characterization of R-loop Structures using Single-Molecule R-loop Footprinting and Sequencing -- Characterization of R-loop Structures using Single-Molecule R-loop Footprinting and Sequencing -- Analyzing RNA-DNA Triplex Formation in Chromatin -- Mapping RNA-chromatin interactions in vivo with RNA-DamID.
520			_aThis volume focuses on RNAs interacting with chromatin and their function. Chapters guide readers through transcription, splicing, non-coding RNA function and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Chromatin Interactions: Methods and Protocols aims to be a starting-point to expand researchers experimental approaches towards the numerous outstanding questions in this new and expanding field.
988			_aSpringer_Protocols_2020
650		7	_2embne _9140562 _aGenética humana
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781071606797
776	0	8	_iPrinted edition: _z9781071606810
776	0	8	_iPrinted edition: _z9781071606827
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0680-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI