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008			230520s2020 xxu| o |||| 0|eng d
020			_a9781071605325
024	7		_a10.1007/978-1-0716-0532-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH205 _b2020 EB
245	0	0	_aSingle Molecule Microscopy in Neurobiology _cedited by Nobuhiko Yamamoto, Yasushi Okada
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIII, 331 páginas) _b94 ilustraciones, 73 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v154
505	0		_aSingle Molecule Imaging of Intracellular Transport in Neurons and Non-Neuronal Cells: From Microscope Optics to Sample Preparations -- In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans -- Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components -- Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of an Activity-Dependent Transcription Factor -- Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons -- Approaching Protein‐Protein Interactions in Membranes using Single Particle Tracking and Packing Coefficient Analysis -- Synaptic Function and Neuropathological Disease Revealed by Quantum-Dot Single Particle Tracking -- Multipolarization Darkfield Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking -- Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons -- Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties -- Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN -- Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons -- Three Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons using Selective Plane Illumination -- A Protocol for Single Molecule Translation Imaging in Xenopus Retinal Ganglion Cells -- Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy.
520			_aThis volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. .
988			_aSpringer_Protocols_2020
650		7	_2embne _9144348 _aNeuronas
650		7	_2embne _9139039 _aNeurobiología
776	0	8	_iPrinted edition: _z9781071605318
776	0	8	_iPrinted edition: _z9781071605332
776	0	8	_iPrinted edition: _z9781071605349
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0532-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI