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020 _a9781071603468
024 7 _a10.1007/978-1-0716-0346-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aSF745
_b2020 EB
245 0 0 _aAnimal Influenza Virus :
_bMethods and Protocols
_cedited by Erica Spackman
250 _a3rd edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XII, 454 páginas)
_b56 ilustraciones, 46 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2123
505 0 _aHemagglutination Assay for Influenza Virus -- Hemagglutination Inhibition Assay -- Immunohistochemical Staining of Influenza Virus in Tissues -- Plasmid-based Reverse Genetics of Influenza A virus -- Antigenic Cartography: Overview and Current Developments -- Complete Genome Sequencing of Influenza A viruses using Next-G\generation Sequencing -- A Brief Introduction to Avian Influenza Virus -- Wild Bird Surveillance for Avian Influenza Virus -- Avian Influenza Virus Sample Types, Collection, and Handling -- Avian Influenza Virus RNA Extraction -- Avian Influenza Virus Detection and Quantitation by Real-time RT-PCR -- Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs -- Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus -- Detection of Influenza A Antibodies in Avian Samples by ELISA -- Conducting Influenza Virus Pathogenesis Studies in Avian Species -- Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry -- Techniques for the Measurement of Cell Mediated Immune Responses to Avian Influenza Virus -- A Brief Introduction to Influenza A Virus in Swine -- Specimen Types, Collection, and Transport for Influenza A Viruses of Swine -- Isolation of Swine Influenza A Virus in Cell Cultures and Embryonated Chicken Eggs -- RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-time RT-PCR -- Enzyme-linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype SpecificIgG and IgA Whole Virus Antibody to Influenza A virus in Swine -- Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine -- Detection and Titration of Influenza A Virus Neuraminidase Inhibiting (NAI) Antibodies using an Enzyme-Linked Lectin Assay (ELLA) -- In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza -- A Brief Introduction to Equine Influenza and Equine Influenza Viruses -- Equine Influenza Diagnosis: Sample Collection and Transport -- RNA Extraction from Equine Samples for Equine Influenza Virus -- Type A Influenza Virus Detection from Horses by Real-Time RT-qPCR and Insulated Isothermal RT-PCR -- Equine Influenza Culture Methods -- Equine Influenza Serological Methods -- Influenza A Viruses in Peridomestic Mammals -- A Brief Introduction to Influenza A Virus in Marine Mammals.
520 _aThis third edition aims to provide new and updated methods on animal influenza viruses as well as more advanced protocols that will guide the reader in designing research. Chapters detail influenza in peridomestic animals, marine mammals, savian influenza, swine influenza, equine influenza, hemagglutination, genome sequencing, and influenza in other mammals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Animal Influenza Virus: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
988 _aSpringer_Protocols_2020
650 7 _2embne
_9673146
_aAnimales
_xEnfermedades
650 7 _2embne
_9164300
_aGripe aviar
776 0 8 _iPrinted edition:
_z9781071603451
776 0 8 _iPrinted edition:
_z9781071603475
776 0 8 _iPrinted edition:
_z9781071603482
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0346-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eIG
_zSI