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008			230517s2020 xxu| o |||| 0|eng d
020			_a9781493999521
024	7		_a10.1007/978-1-4939-9952-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK898 .C34 _b2020 EB
245	0	0	_aPlant and Food Carotenoids : _bMethods and Protocols _cedited by Manuel Rodríguez-Concepción, Ralf Welsch
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XV, 421 páginas) _b81 ilustraciones, 58 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2083
505	0		_aPathways for Carotenoid Biosynthesis, Degradation, and Storage -- A Simple in vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-chain Prenyltransferases -- Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase in vitro -- Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO -- Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases -- Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases -- Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters -- Applications of Visible Spectroscopy and Colour Measurements in the Assessments of Carotenoid Levels in Foods -- A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids -- Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management -- cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues -- Determination of Plant Volatile Apocarotenoids -- A Method for Extraction and LC-MS Based Identification of Carotenoid-Derived Dialdehydes in Plants -- Fractionation of Tomato Fruit Chromoplasts -- Quantification of Strigolactones -- Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS -- Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy -- Visualization of Carotenoid-storage Structures in Fruits by Transmission Electron Microscopy -- Light Microscopy and Raman Imaging of Carotenoids in Plant Cells in situ and in Released Carotene Crystals -- Carotenoid Isotopologue Profiling in 13C-labeled Leaf Extracts by LC-MS and LC-FTICR-MS -- Quantification of Carotenoid Pathway Flux in Green and Non-green Systems -- Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis -- Use of Potyvirus Vectors to Produce Carotenoids in Plants -- Agrobacterium tumefaciens Mediated Stable Transformation of Daucus carota -- CRISPR-Cas-Mediated Gene Knockout in Tomato -- Modulation of Activity of a Carotenoid Pathway Through the use of the TET-on Regulatory System: Application in the Fungus Fusarium fujikuroi -- Intervention Studies in Humans -- In vitro Digestion for Control and Monitoring of Food Effects In Relation To Micellarization Index of Carotenoids -- Evaluation of Carotenoids Protection against Oxidative Stress in the Animal Model Caenorhabditis elegans -- Mouse Models to Study Antiobesogenic Effects of Carotenoids.
520			_aThis volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant and Food Carotenoids: Methods and Protocols aims to be helpful to researchers of other disciplines that are impacted by carotenoids, including photosynthesis, biotechnology, food science, and nutrition.
988			_aSpringer_Protocols_2020
650		7	_2embne _9160173 _aCarotenoides
776	0	8	_iPrinted edition: _z9781493999514
776	0	8	_iPrinted edition: _z9781493999538
776	0	8	_iPrinted edition: _z9781493999545
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9952-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI