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008			230517s2018 xxu| o |||| 0|eng d
020			_a9781493988020
024	7		_a10.1007/978-1-4939-8802-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC933 _b2018 EB
245	0	0	_aRheumatoid Arthritis : _bMethods and Protocols _cedited by Shuang Liu
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (IX, 253 páginas) _b59 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1868
505	0		_aCollagen-induced Arthritis Models -- Human Xenograft Model -- Long-term Constant Subcutaneous Drug Administration -- Clinical Scoring of Disease Activity in Animal Models -- Histological Analysis of Arthritic Joints -- Preparation of Joint Extracts -- Production of Immunizing Antigen Proteoliposome Using Cell-free Protein Synthesis System -- Re-construction of Protein/Liposome Complex -- Production of Neutralizing Antibody -- Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen -- Generation of Specific Aptamers -- Production of Lentiviral Particles -- RNA Interference Ex Vivo -- Lentiviral-mediated Systemic RNA Interference in vivo -- Mesenchymal Stem Cell Engineering -- Screening of Ca2+ Influx in Lymphocytes -- Single-cell Ca2+ Imaging -- Electrophysiological Methods to Measure Ca2+ Current -- The Functional Assessment of T cells -- Release of Antibodies and Cytokines from B cells -- Evaluation of Autoreactive Responses -- Bone-resorption Activity in Mature Osteoclasts -- Animal Models of Vasculitis -- Design an Intervention Study -- Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis.
520			_aThis volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.
988			_aSpringer_Protocols_2018
650		7	_2embne _9145879 _aArtritis reumatoide
776	0	8	_iPrinted edition: _z9781493988013
776	0	8	_iPrinted edition: _z9781493988037
776	0	8	_iPrinted edition: _z9781493993932
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8802-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI