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008			230517s2018 xxu| o |||| 0|eng d
020			_a9781493986484
024	7		_a10.1007/978-1-4939-8648-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.65 .I49 _b2018 EB
245	0	0	_aAntibody Engineering : _bMethods and Protocols _cedited by Damien Nevoltris, Patrick Chames
250			_a3rd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XVI, 543 páginas) _b106 ilustraciones, 73 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1827
505	0		_aAntibody Design and Humanization via In Silico Modeling -- Antibody Affinity Maturation by Computational Design -- Use of IMGT® Databases and Tools for Antibody Engineering and Humanization -- Construction of Human Naïve Antibody Gene Libraries -- Construction of Synthetic Antibody Libraries -- Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments -- Display Technologies for Generation of Ig Single Variable Domains -- A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries -- Phage Display and Selections on Purified Antigens -- Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display -- Selection of Antibody Fragments against Structured DNA by Phage Display -- Selection of Antibody Fragments by Yeast Display -- Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display -- In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display -- Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood -- Expression of IgG Monoclonals with Engineered Immune Effector Functions -- An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines -- Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins -- Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases -- Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity -- Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0 -- Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance -- Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development -- The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies -- Selection and Use of Intracellular Antibodies -- Site-Specific Radioactive Labeling of Nanobodies.
520			_aThis detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.
988			_aSpringer_Protocols_2018
650		7	_2embne _9405730 _aAnticuerpos monoclonales
650		7	_2embne _9138044 _aGenética
776	0	8	_iPrinted edition: _z9781493986477
776	0	8	_iPrinted edition: _z9781493986491
776	0	8	_iPrinted edition: _z9781493993536
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8648-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI