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008			230516s2018 xxu| o |||| 0|eng d
020			_a9781493985852
024	7		_a10.1007/978-1-4939-8585-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP97 _b2018 EB
245	0	0	_aPlatelets and Megakaryocytes _nVolume 4, _pAdvanced Protocols and Perspectives _cedited by Jonathan M. Gibbins, Martyn Mahaut-Smith
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 290 páginas) _b72 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1812
505	0		_aImmobilisation of Non-activated Unfixed Platelets for Real Time Single-cell Analysis -- Imaging Platelets and Megakaryocytes by High Resolution Laser Fluorescence Microscopy -- Single Molecule Localisation and Structured Illumination Microscopy of Platelet Proteins -- Electron Tomography and Correlative Approaches in Platelet Studies -- Screening and High-throughput Platelets Assays -- High-throughput Signaling Profiling in Blood Platelets by Multiplexed Phosphoflow Cytometry -- Precise Quantification of Platelet Proteins and their Phosphorylation States -- The Study of Platelet Receptors using Artificial Lipid Bilayers -- Three Dimensional Culture in a Methylcellulose-based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation -- Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor Driven Forward Programming -- Three-dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production -- Fluorescence Approaches to Image and Quantify the Demarcation Membrane System in Living Megakaryocytes -- High-resolution 3D Imaging of Megakaryocytes using Focused Ion Beam-Scanning Electron Microscopy -- Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-sheet Fluorescence Microscopy -- Mathematical Techniques for Understanding Platelet Regulation and the Development of New Pharmacological Approaches.
520			_aThe main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or avoiding known pitfalls. Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.
988			_aSpringer_Protocols_2018
650		7	_2embne _9677737 _aPlaquetas sanguíneas
650		7	_2embne _9242082 _aHistología _xTécnica
776	0	8	_iPrinted edition: _z9781493985845
776	0	8	_iPrinted edition: _z9781493985869
776	0	8	_iPrinted edition: _z9781493993345
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8585-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI