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008			230516s2018 xxu| o |||| 0|eng d
020			_a9781493987993
024	7		_a10.1007/978-1-4939-8799-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .Z55 _b2018 EB
245	0	0	_aZinc Finger Proteins : _bMethods and Protocols _cedited by Jia Liu
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 276 páginas) _b59 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1867
505	0		_aPart 1. Basic Biology and Engineering of ZFPs -- PRDM14, a zinc finger protein, regulates cancer stemness -- Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins -- Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation -- Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology -- Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers -- Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins -- Directed evolution of targeted recombinases for genome engineering -- Optical recording of cellular zinc dynamics with zinc finger based biosensors -- Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins -- Genome editing of MSCs as a platform for cell therapy -- Part 2. Methods for the Evaluation and Prevention of ZFN Cytotoxicity -- Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells -- Selection and characterization of DNA aptamers against FokI nuclease domain -- An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs -- Part 3. Delivery Methods of ZFPs -- Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering -- Gene editing in channel catfish via double electroporation of zinc finger nucleases -- Delivery of mtZFNs into early mouse embryos -- Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain -- In-vivo applications of cell-penetrating zinc finger transcription factors -- Manufacturing and delivering genome-editing proteins.
520			_aThis detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.
988			_aSpringer_Protocols_2018
650		7	_2embne _9669891 _aProteínas _xEstructura
650		7	_2embne _9672504 _aCinc
776	0	8	_iPrinted edition: _z9781493987986
776	0	8	_iPrinted edition: _z9781493988006
776	0	8	_iPrinted edition: _z9781493993925
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8799-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI