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008			230513s2007 xxu| o |||| 0|eng d
020			_a9781597453622
024	7		_a10.1007/978-1-59745-362-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH324.9.C7 _b2007 EB
245	0	0	_aCryopreservation and Freeze-Drying Protocols _cedited by John G. Day, Glyn Stacey
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XI, 347 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v368
505	0		_aLong-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers -- The Principles of Freeze-Drying -- Principles of Cryopreservation -- Lyophilization of Proteins -- Vacuum-Drying and Cryopreservation of Prokaryotes -- Freeze-Drying of Yeast Cultures -- Cryopreservation of Yeast Cultures -- Freeze-Drying Fungi Using a Shelf-Freeze Drier -- Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying -- Cryopreservation of Microalgae and Cyanobacteria -- Cryopreservation of Plant Cell Suspensions -- Cryopreservation of Shoot Tips and Meristems -- Cryopreservation of Desiccation-Tolerant Seeds -- Cryopreservation of Fish Sperm -- Cryopreservation of Avian Spermatozoa -- Cryopreservation of Animal and Human Cell Lines -- Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use -- Cryopreservation of Human Embryonic Stem Cell Lines -- Cryopreservation of Primary Animal Cell Cultures -- Cryopreservation of Red Blood Cells and Platelets -- Cryopreservation of Mammalian Semen -- Cryopreservation of Mammalian Oocytes -- Cryopreservation of Mammalian Embryos.
520			_aCryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.
988			_aSpringer_Protocols_2007
650		7	_2embne _9673643 _aCrioconservación de órganos, tejidos, etc.
650		7	_2embne _9688493 _aLiofilización
776	0	8	_iPrinted edition: _z9781617375286
776	0	8	_iPrinted edition: _z9781588293770
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-362-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI