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008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592592913
024	7		_a10.1385/1592592910 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR184.315 _b2003 EB
245	0	0	_aMHC Protocols _cedited by Stephen H. Powis, Robert W. Vaughan
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XII, 340 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v210
505	0		_aDatabases -- HLA Informatics -- Accessing HLA Sequencing Data Through the 6ace Database -- Polymorphism in Classical and Nonclassical Hla Genes -- HLA Typing by Restriction Fragment Length Polymorphism Analysis -- PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles -- PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR -- HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes -- PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles -- HLA Typing With Reference Strand-Mediated Conformation Analysis -- Sequencing Protocols for Detection of HLA Class I Polymorphism -- HLA-E and HLA-G Typing -- Typing Alleles of HLA-DM -- Polymorphism in Non-Hla Mhc Genes -- Typing Alleles of TAP1 and TAP2 -- Determining Alleles of the C2 Gene by Southern Blotting -- Complement C4 Protein and DNA Typing Methods -- Typing of Tumor Necrosis Factor Alleles -- Molecular Typing of the MHC Class I Chain-Related Gene Locus -- Microsatellites -- HLA Microsatellite Analysis.
520			_aMany of the genes found in the human major histocompatibility complex (MHC:HLA region) are subject to extreme polymorphism. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. Many MHC genes also have significant disease association and play a pivotal role in the control of immune response. In MHC Protocols, leading experts detail readily reproducible methods for identifying these polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP2, C2, C4, and TNF-a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles. Each method is written by an investigator who has used the technique extensively and includes troubleshooting tips and notes on pitfalls to avoid. Comprehensive and highly practical, MHC Protocols offers both experienced and novice investigators not only a state-of-the-art overview of this dramatically evolving area of immunology, but also a reliable guide to the identification of MHC gene polymorphisms.
988			_aSpringer_Protocols_2003
650		7	_2embne _9155268 _aPolimorfismo genético
650		7	_2embne _9688492 _aInmunogenética
776	0	8	_iPrinted edition: _z9781489938206
776	0	8	_iPrinted edition: _z9780896035485
776	0	8	_iPrinted edition: _z9781489938190
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592910 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI