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020 _a9781592590551
024 7 _a10.1385/1592590551
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP620
_b2001 EB
245 1 0 _aCapillary Electrophoresis of Nucleic Acids
_cedited by Keith R. Mitchelson, Jing Cheng
250 _a1st edition 2001
264 1 _aTotowa, NJ
_bHumana Press
_c2001
300 _a1 recurso en línea (XVI, 484 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v162
505 0 _aAnalytical Considerations for Capillary Electrophoresis -- Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis -- DNA Separation Mechanisms During Electrophoresis -- Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis -- Quantitative Measurements -- Microchip-Based Capillary Electrophoresis Systems -- DNA Sequencing by Capillary Array Electrophoresis -- 7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis -- Capillary Matrix: Considerations for Capillary Electrophoresis -- Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA -- Capillary Coatings -- Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis -- Sieving Matrix Selection -- Parameters Affecting Capillary Electrophoretic Separation of DNA -- Electrophoresis Matrix: Applications for DNA Fragment Sizing -- DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals -- Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material -- DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium -- 16 DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries -- Capillary Electrophoresis with Glycerol as an Additive -- Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels -- Practical Applications for Large DNA Fragment Sizing and Fragment Collection -- Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments -- Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments -- Collection of Capillary Electrophoresis Fractions on a Moving Membrane -- Separation of Supercoiled DNA Using Capillary Electrophoresis -- Practical Applications for Oligonucleotide and Nucleotide Analysis -- Quality Control of Nucleotides and Primers for PCR -- Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis -- Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides -- Capillary Electrophoresis Separation of Ribonucleosides -- On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries -- Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA -- Capillary Electrophoresis-Mass Spectrometric Analysis of DNA Adducts -- Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis -- Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis -- Analysis of DNA Damage Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry -- Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Sieving Electrophoresis -- Capillary Electrophoresis of Urinary Normal and Modified Nucleosides of Cancer Patients.
520 _aThe advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This first volume of the set, Introduction to the Capillary Electrophoresis of Nucleic Acids, covers the practical and theoretical considerations behind the use of capillary electrophoresis for the analysis of small oligonucleotides and modified nucleotides. Along with detailed instructions ensuring ready reproducibility, these protocols offer time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE. Several chapters are devoted to the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides by CE. The companion volume, Practical Applications of Capillary Electrophoresis, addresses techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions. Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.
988 _aSpringer_Protocols_2001
650 7 _2embne
_9688491
_aElectroforesis capilar
650 7 _2embne
_9140525
_aÁcidos nucleicos
700 1 _aMitchelson, Keith R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aCheng, Jing
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617371868
776 0 8 _iPrinted edition:
_z9780896037793
776 0 8 _iPrinted edition:
_z9781489939029
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590551
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eIG
_zSI