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020 _a9781603270298
024 7 _a10.1007/978-1-60327-029-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aFree Radicals and Antioxidant Protocols
_cedited by Rao M. Uppu, Subramanyam N. Murthy, William A. Pryor, Narasimham L. Parinandi.
250 _a2nd edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (XVI, 480 páginas)
_b115 ilustraciones, 11 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v610
505 0 _aReactive Oxygen and Nitrogen Species -- In Vivo Imaging of Free Radicals and Oxygen -- In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle, Lithium Phthalocyanine -- Measurement of Plasma Nitrite by Chemiluminescence -- Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species -- Natural and Synthetic Antioxidants -- Phenolic Acids and Flavonoids: Occurrence and Analytical Methods -- Design, Synthesis, and Action of Antiatherogenic Antioxidants -- Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity Against Lipid Peroxidation -- Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy -- Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes -- Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo -- Cellular Oxidative Stress -- The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia -- A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling -- Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction -- Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids -- Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening -- Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic Neurons Exposed to Cholesterol Secoaldehyde -- Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration -- Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension -- DNA Oxidation, Oxidative Lipidomics, and Biomarkers -- A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA -- Analysis of Urinary 8-oxo-7,8-dihydro-2?-deoxyguanosine by Liquid Chromatography-Tandem Mass Spectrometry -- Oxidative Lipidomics of Apoptosis: Quantitative Assessment of Phospholipid Hydroperoxides in Cells and Tissues -- Simultaneous Analysis of Multiple Lipid Oxidation Products In Vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS) -- Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides -- Oxidized Low-Density Lipoprotein -- Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease -- Enzyme Immunoassay of Isoprostanes -- Application of Membrane Extraction with Sorbent Interface for Breath Analysis.
520 _aIn recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.
700 1 _aUppu, Rao M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMurthy, Subramanyam N
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aPryor, William A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aParinandi, Narasimham L
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607614913
776 0 8 _iPrinted edition:
_z9781588297105
776 0 8 _iPrinted edition:
_z9781617796838
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-029-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2010
999 _c391025
_d391025