| 000 | 05159nam a22003735i 4500 | ||
|---|---|---|---|
| 001 | 391021 | ||
| 003 | ES-MaUEC | ||
| 005 | 20230102122444.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 121026s2013 xxu| s |||| 0|eng d | ||
| 020 | _a9781627031554 | ||
| 024 | 7 |
_a10.1007/978-1-62703-155-4 _2doi |
|
| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
||
| 245 | 1 | 0 |
_aPeroxisome Proliferator-Activated Receptors (PPARs) _bMethods and Protocols _cedited by Mostafa Z. Badr, Jihan A. Youssef. |
| 250 | _a1st edition 2013 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c2013 |
|
| 300 |
_a1 recurso en línea (XI, 343 páginas) _b65 ilustraciones, 37 ilustraciones a color |
||
| 336 |
_atexto _btxt _2rdacontent |
||
| 337 |
_aelectrónico _bc _2rdamedia |
||
| 338 |
_arecurso electrónico _bcr _2rdacarrier |
||
| 347 |
_aarchivo de texto _bPDF |
||
| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v952 |
|
| 505 | 0 | _aPPARs: History and Advances -- PPAR-Alpha Cloning, Expression and Characterization -- PPARGC1A and PPARG Genotyping. Beginner's Guide to Genotyping with Unlabeled Probes -- Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPARβ/d Overexpression -- Specific knockdown of PPARd Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering -- Dominant-Negative and Knockdown Approaches to Studying PPAR activity -- Producing PPARgamma2 Knockdown in Mouse Liver -- Adipose Tissue-Specific PPARg Gene Targeting -- Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs -- PPAR SUMOylation: Some Useful Experimental Tips -- Analyzing Phosphorylation-Dependent Regulation of Subcellular Transcriptional Activity of Transcriptional Coactivator NT-PGC-1a -- In vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses -- FISH Analysis Using PPARg Specific Probes for Detection of PAX8-PPARg Translocation in Follicular Thyroid Neoplasms -- Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin-Embedded Tissue Sections -- Determination of PPAR Expression by Western-Blot -- Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators -- Estimation of the PPARa Agonism of Fibrates by a Combined MM-Docking Approach -- Combined Biophysical and Cell-based Approaches for the Assessment of Ligand Binding to PPARg -- Exploring PPAR Modulation in Experimental Mice -- Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold -- Analyzing PPAa/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry -- Synthesis, Mass Spectrometric Characterization, and Analysis of the PPARd Agonist GW1516 and its Major Human Metabolites - Targets in Sports Drug Testing -- LC-MS-Based Method for the Qualitative and Quantitative Analysis of the Novel PPARg Agonist KR-62980 -- Behavioral Paradigms to Evaluate PPAR Modulation in Animal Models of Brain Injury. | |
| 520 | _aAll three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are currently underway to fully characterize their functioning and to develop safer and more effective PPAR modulators to treat a myriad of diseases and conditions. In Peroxisome proliferator-Activated Receptors: Methods and Protocols, renowned experts in the PPAR arena provide detailed protocols for investigating these receptors. Chapters contain methods ranging from the cloning of receptors to their knockdown, to protocols exploring posttranslational modifications of PPARs and coactivators, as well as receptor subcellular localization. Also assembled are methods to evaluate the involvement of these receptors in behavior functions, an emerging facet in PPAR research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. With its well-honed methodologies, Peroxisome proliferator-Activated Receptors: Methods and Protocols, will be a useful resource for all seeking to advance their knowledge of this field. | ||
| 700 | 1 |
_aBadr, Mostafa Z _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 700 | 1 |
_aYoussef, Jihan A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 776 | 0 | 8 |
_iPrinted edition: _z9781627031561 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781627031547 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493962570 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-155-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
| 942 |
_2lcc _cLE |
||
| 988 | _aSpringer_Protocols_2013 | ||
| 999 |
_c391021 _d391021 |
||