000			04308nam a22003615i 4500
001			391008
003			ES-MaUEC
005			20230102122440.0
007			cr nn 008mamaa
008			160908s2016 xxu| s |||| 0|eng d
020			_a9781493963584
024	7		_a10.1007/978-1-4939-6358-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSUMO _bMethods and Protocols _cedited by Manuel S. Rodriguez.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 306 páginas) _b54 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1475
505	0		_aConcepts and Methodologies to Study Protein SUMOylation: An Overview -- The Regulation of Chromatin by Dynamic SUMO Modifications -- Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex -- Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria -- A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO Conjugation -- Identification and Characterization of SUMO-SIM Interactions -- Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties -- Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins -- In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific Proteases -- Detection of Protein SUMOylation In Situ by Proximity Ligation Assays -- In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized Cells -- Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO Strategy -- Label-Free Identification and Quantification of SUMO Target Proteins -- The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteins -- Isolation of In Vivo SUMOylated Chromatin-Bound Proteins -- Identification of Substrates of Protein-Group SUMOylation -- Tools to Study SUMO Conjugation in Caenorhabditis elegans -- Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry Analysis -- Detection of SUMOylation in Plasmodium falciparum -- Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.
520			_aThis volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls. Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.
700	1		_aRodriguez, Manuel S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493963560
776	0	8	_iPrinted edition: _z9781493963577
776	0	8	_iPrinted edition: _z9781493981755
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6358-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c391008 _d391008