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020 _a9781071615188
024 7 _a10.1007/978-1-0716-1518-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR47
_b2021 EB
245 0 4 _aThe Oral Microbiome :
_bMethods and Protocols
_cedited by Guy R. Adami
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XIII, 295 páginas)
_b122 ilustraciones, 37 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2327
505 0 _aIncreasing Reproducibility in Oral Microbiome Research -- Oral Sampling Techniques -- Functional Metagenomic Screening for Antimicrobial Resistance in the Oral Microbiome -- Isolation and Functional Characterization of Fusobacterium nucleatum Bacteriophage -- Comparison of Microbiome in Stimulated Saliva in Edentulous and Dentate Subjects -- Host DNA Depletion in Saliva Samples for Improved Shotgun Metagenomics -- A Standardized Approach for Shotgun Metagenomic Analysis of Ancient Dental Calculus -- Whole-Genome Sequencing of Pathogens in Saliva: A Target-Enrichment Approach for SARS-CoV-2 -- Assessing the Relationship Between Nitrate-Reducing Capacity of the Oral Microbiome and Systemic Outcomes -- Identification of Oral Bacterial Biosynthetic Gene Clusters Associated with Caries -- Usage of Metatranscriptomics to Understand Oral Disease -- Noninvasive Acquisition of Oral Mucosal Epithelial miRNA and Bacteria DNA/RNA from a Single Site -- Bottom-Up Community Proteome Analysis of Saliva Samples and Tongue Swabs by Data-Dependent Acquisition Nano LC-MS/MS Mass Spectrometry -- Strain Level Profiling of Oral Microbiota with Targeted Sequencing -- Profiling the Human Oral Mycobiome in Tissue and Saliva Using ITS2 DNA Metabarcoding Compared to a Fungal Specific Database -- Measuring Effects of Dietary Fiber on the Murine Oral Microbiome with Enrichment of 16S rDNA Prior to Amplicon Synthesis -- Antibiotic Conditioning and Single Gavage Allows Stable Engraftment of Human Microbiota in Mice. .
520 _aThis volume discusses the oral microbiome, and oral and systemic health. The chapters in this book cover topics such as analytical techniques for identifying and measuring oral bacteria; strategies for controlling common sources of variability in oral microbiome methods for viral bacterial and fungal analysis; ways to study oral DNA and RNA samples to identify molecular pathways to disease; approaches to functional assays for oral bacteriophage, antibiotic purging of systemic bacteria; and metaproteomic analysis of various oral samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, The Oral Microbiome: Methods and Protocols is a valuable resource for any scientist or researcher looking to further study this exciting and developing field.
988 _aSpringer_Protocols_2021
650 7 _2embne
_9168146
_aMicrobiología oral
650 7 _2embne
_9169178
_aBoca
_xEnfermedades
776 0 8 _iPrinted edition:
_z9781071615171
776 0 8 _iPrinted edition:
_z9781071615195
776 0 8 _iPrinted edition:
_z9781071615201
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1518-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eb
_zSI