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008			200131s2020 xxu| o |||| 0|eng d
020			_a9781071602904
024	7		_a10.1007/978-1-0716-0290-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP110 .G45 _b2020 EB
245	0	0	_aRNA Interference and CRISPR Technologies : _bTechnical Advances and New Therapeutic Opportunities _cedited by Mouldy Sioud
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XVI, 486 páginas) _b70 ilustraciones, 48 ilustraciones a color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2115
505	0		_aRNA and CRISPR Interferences: Past, Present and Future Perspectives -- Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents -- Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo -- Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy -- PAMAM Dendrimers as a Delivery System for Small Interfering RNA -- Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo -- Optimized siRNA Delivery into Primary Immune Cells using Electroporation -- Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference -- Exploring 5'-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency -- In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector -- Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors -- MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302 -- Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers -- Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference -- Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference -- Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy -- Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing -- Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency -- CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity -- CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly -- Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task -- Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted? -- Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy -- CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells -- Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology -- Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation -- CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation.
520			_aThis volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. .
988			_aSpringer_Protocols_2020
650		7	_2embne _9162770 _aExpresión génica
650		7	_2embne _9138044 _aGenética
776	0	8	_iPrinted edition: _z9781071602898
776	0	8	_iPrinted edition: _z9781071602911
776	0	8	_iPrinted edition: _z9781071602928
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0290-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI