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008			200123s2020 xxu| o |||| 0|eng d
020			_a9781071603420
024	7		_a10.1007/978-1-0716-0342-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK665 _b2020 EB
245	0	0	_aPlant Embryogenesis : _bMethods and Protocols _cedited by Martin Bayer
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 298 páginas) _b98 ilustraciones, 84 ilustraciones a color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2122
505	0		_aGenetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize -- EMS Mutagenesis of Arabidopsis Seeds -- EMS Mutagenesis of Maize Pollen -- Live-cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana -- Analyzing Sub-Cellular Reorganization during Early Arabidopsis Embryogenesis using Fluorescent Markers -- From Stained Plant Tissues to Quantitative Cell Segmentation Analysis with MorphoGraphX -- Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos -- Manual Isolation of Living Early Embryos from Tobacco Seeds -- Profiling Transcriptomes of Manually Dissected Arabidopsis Embryos -- Laser-assisted Microdissection of Plant Embryos for Transcriptional Profiling -- Cell-type-specific Transcriptomics in the Plant Embryo Using an Adapted INTACT Protocol -- Transcriptomic Profiling of the Arabidopsis Embryonic Epidermis Using FANS in Combination with RNAseq -- Visualizing Protein Associations in living Arabidopsis Embryo -- Imaging of Embryo Sac and Early Seed Development in Maize after Feulgen Staining -- Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis -- Microscopical Detection of Cell Death Processes during Scots Pine Zygotic Embryogenesis -- Regulation of Somatic Embryo Development in Norway Spruce -- In vitro Production of Zygotes by Electro-Fusion of Rice Gametes -- Isolated Microspore Culture in Brassica napus -- Anther Culture in Eggplant (Solanum melongena L.). .
520			_aThis volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field. The chapter "Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2020
650		7	_2embne _9668339 _aEmbriología vegetal
650		7	_2embne _9138492 _aBotánica
776	0	8	_iPrinted edition: _z9781071603413
776	0	8	_iPrinted edition: _z9781071603437
776	0	8	_iPrinted edition: _z9781071603444
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0342-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI