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008			191230s2020 xxu| o |||| 0|eng d
020			_a9781071602317
024	7		_a10.1007/978-1-0716-0231-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH431 _b2020 EB
245	0	0	_aRNA Chaperones : _bMethods and Protocols _cedited by Tilman Heise
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 314 páginas) _b78 ilustraciones, 70 ilustraciones a color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2106
505	0		_aBiochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones -- Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy -- Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity -- Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding -- Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein's RNA Chaperone Activity -- Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function -- Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La -- Kinetic and Thermodynamic Analyses of RNA-Protein Interactions -- Detection of MicroRNAs Released from Argonautes -- Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE-Chemical Probing -- RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing -- In Vivo RNA Chemical Footprinting Analysis in Archaea -- RNA Structure Analysis by Chemical Probing with DMS and CMCT -- Disordered RNA Binding Region Prediction with DisoRDPbind -- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci -- Site-Specific Dual-Color Labeling of Long RNAs -- Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding -- Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy -- Isolation and Analysis of Bacterial Ribosomes through Sucrose Gradient Ultracentrifugation.
520			_aThis book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones. Chapter 3 is available Open Access under a CC-BY 4.0 license via link.springer.com.
988			_aSpringer_Protocols_2020
650		7	_2embne _9140562 _aGenética humana
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781071602300
776	0	8	_iPrinted edition: _z9781071602324
776	0	8	_iPrinted edition: _z9781071602331
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0231-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI