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008			180914s2018 xxu| o |||| 0|eng d
020			_a9781493987665
024	7		_a10.1007/978-1-4939-8766-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH431 _b2018 EB
245	0	0	_aX-Chromosome Inactivation : _bMethods and Protocols _cedited by Takashi Sado
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 251 páginas) _b53 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1861
505	0		_aScreening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles.
520			_aThis book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.
988			_aSpringer_Protocols_2018
650		7	_2embne _9140562 _aGenética humana
650		7	_2embne _9139992 _aCromosomas
776	0	8	_iPrinted edition: _z9781493987658
776	0	8	_iPrinted edition: _z9781493987672
776	0	8	_iPrinted edition: _z9781493993864
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8766-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI